Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Improved method for fluorescence cytometric immunohematology testing.

John D Roback1, Sheilagh Barclay, Christopher D Hillyer

  • 1Department of Pathology and Laboratory Medicine, Emory Univerity School of Medicine, Atlanta, Georgia 30322, USA. jroback@emory.edu

Transfusion
|February 14, 2004
PubMed
Summary

A modified fluorescence cytometry (FC) method enhances immunohematology testing accuracy and sensitivity for red blood cell (RBC) antigen and antibody detection. This improved FC assay is comparable to standard methods and suitable for automation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Point-of-care GlycoStrips detect serum and salivary antibodies to blood group antigens and pathogens.

Trends in biotechnology·2026
Same author

Exploratory comparison of PASC and SARS-CoV-2 infection through metabolomics and lipidomics in early pandemic and Omicron-era.

Biochemistry and biophysics reports·2026
Same author

Red blood cell transfusion survival in sickle cell disease is reduced by donor characteristics and recipient spleen activity.

Blood advances·2026
Same author

Prevalence of CMV DNA in plasma from United States blood donors.

Transfusion·2025
Same author

A Thymus-Independent Artificial Organoid System Supports Complete Thymopoiesis from Rhesus Macaque-Derived Hematopoietic Stem and Progenitor Cells.

Biomedicines·2025
Same author

Mitofusin agonists enhances long-term engraftment and potency of HSC cultures <i>in vivo</i>.

bioRxiv : the preprint server for biology·2025

Area of Science:

  • Immunohematology
  • Flow Cytometry
  • Clinical Diagnostics

Background:

  • Previous fluorescence cytometry (FC) methods for immunohematology were limited by manual processes like vacuum filtration and standard flow cytometers.
  • These limitations hindered the integration of FC into automated laboratory platforms.

Purpose of the Study:

  • To develop and evaluate a modified FC procedure for accurate and automated immunohematology testing.
  • To assess the performance of the modified FC method compared to column agglutination technology (CAT).

Main Methods:

  • A modified FC procedure using low-speed centrifugation for red blood cell (RBC) staining in 96-well plates was developed.
  • Data acquisition was performed using benchtop capillary cytometers (PCA and PCA-96).
  • Clinical samples were tested for ABO/D antigen and RBC alloantibodies, with results compared to CAT and tube methods.

Related Experiment Videos

Main Results:

  • The modified FC method demonstrated comparable accuracy to CAT for ABO/D typing (98.7% vs. 98.8%) and alloantibody detection (98.6% vs. 96.3%).
  • FC showed superior sensitivity in detecting weak anti-A and alloantibodies compared to CAT (p < 0.0001).
  • The PCA-96 system achieved high accuracy for ABO/D typing (98.7%) and antibody screening (99.1%).

Conclusions:

  • The improved FC method offers comparable accuracy to CAT with enhanced sensitivity for weak antibodies and mixed-field reactions.
  • The method's compatibility with automated plate-handling systems (PCA-96) facilitates the development of fully automated immunohematology platforms.
  • FC provides a reproducible and sensitive alternative for red blood cell testing.