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Updated: Aug 16, 2026

Steady-state, Pre-steady-state, and Single-turnover Kinetic Measurement for DNA Glycosylase Activity
Published on: August 19, 2013
The condition for pseudo-first-order kinetics in enzymatic reactions is independent of the initial enzyme
1Centre for Mathematical Biology, Mathematical Institute, 24-29 St Giles', Oxford OX1 3LB, UK. schnell@maths.ox.ac.uk
Abstract:
The linearization of the Michaelis-Menten reaction by pseudo-first-order kinetics is revised. A phase-plane analysis allows the derivation of a new condition for its validity that is directly linked to the reaction efficiency, and contrary to widely established knowledge, is independent of the initial enzyme concentration.
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