Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Recombinant allergens for analysing T-cell responses.

Wayne R Thomas1, Belinda J Hales, Wendy-Anne Smith

  • 1Centre for Child Health Research, Telethon Institute for Child Health Research, University of Western Australia, P.O. Box 855, West Perth, Western Australia 6872, Australia. wayne@ichr.uwa.edu.au

Methods (San Diego, Calif.)
|February 14, 2004
PubMed
Summary

Recombinant allergens enable reproducible studies of T-cell responses in allergies. This research details producing recombinant Der p 2, a key house dust mite allergen, for accurate allergy research.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Linking the westernised oropharyngeal microbiome to the immune response in Chinese immigrants.

Allergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology·2020
Same author

Toll-like receptor signalling has inverted U-shaped response over time with the Western environment.

Allergy·2020
Same author

Differential Gene Expression of Lymphocytes Stimulated with Rhinovirus A and C in Children with Asthma.

American journal of respiratory and critical care medicine·2020
Same author

Effects of Ser47-Point Mutation on Conformation Structure and Allergenicity of the Allergen of Der p 2, a Major House Dust Mite Allergen.

Allergy, asthma & immunology research·2018
Same author

A marked shift in innate and adaptive immune response in chinese immigrants living in a western environment.

Allergy·2018
Same author

WHO/IUIS Allergen Nomenclature: Providing a common language.

Molecular immunology·2018

Area of Science:

  • Immunology
  • Allergy Research

Background:

  • T-cell responses are crucial in allergic diseases and Th1/Th2 cytokine pathway studies.
  • Traditional allergen extracts have variable compositions, limiting reproducibility.
  • Recombinant allergens offer a standardized approach to studying allergic responses.

Purpose of the Study:

  • To evaluate the utility of recombinant allergens for studying T-cell responses in allergic individuals.
  • To demonstrate the production of a specific, highly reactive recombinant allergen (Der p 2) from house dust mites.
  • To establish a reproducible method for allergy research using defined allergen components.

Main Methods:

  • Utilizing recombinant allergens for dose-dependent T-cell stimulation assays.
  • Comparing T-cell responses from allergic and non-allergic subjects.

Related Experiment Videos

  • Employing PCR and cDNA production for recombinant allergen synthesis.
  • Producing recombinant Der p 2 allergen from natural sources.

    • Main Results:

    • Purified recombinant allergens elicit expected T-cell responses, validating their use in research.
    • Recombinant allergens allow for reproducible and dose-dependent analysis of immune reactions.
    • Production of reactive recombinant Der p 2 from natural sources was successfully achieved.

    Conclusions:

    • Recombinant allergens are valuable tools for reproducible allergy research, overcoming limitations of natural extracts.
    • Standardized recombinant allergens facilitate accurate verification of T-cell responses and their link to allergic sensitization.
    • The described method enables the production of specific recombinant allergens, advancing the study of allergic mechanisms.