Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Provocation testing with recombinant allergens.

M van Hage-Hamsten1, G Pauli

  • 1Department of Medicine, Division of Clinical Immunology and Allergy, Karolinska Institutet and Hospital, Stockholm, Sweden.

Methods (San Diego, Calif.)
|February 14, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Test methods for estimating the efficacy of the fast-acting disinfectant peracetic acid on surfaces of personal protective equipment.

Journal of applied microbiology·2017
Same author

EAACI Molecular Allergology User's Guide.

Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology·2016
Same author

Characterization of profilin and polcalcin panallergens from ash pollen.

Journal of investigational allergology & clinical immunology·2014
Same author

Identification of enolases and aldolases as important fish allergens in cod, salmon and tuna: component resolved diagnosis using parvalbumin and the new allergens.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2013
Same author

[Cross reactions between pollens and vegetable food allergens].

Revue des maladies respiratoires·2013
Same author

Molecular allergology in practice: an unusual case of LTP allergy.

European annals of allergy and clinical immunology·2012

Recombinant allergens, produced using DNA technology, offer precise diagnostics for allergies. In vivo testing of these molecules is crucial before their use in immunotherapy.

Area of Science:

  • Allergen immunotherapy
  • Molecular biology
  • Diagnostic immunology

Background:

  • DNA technology allows for the creation of defined recombinant allergen molecules.
  • Recombinant allergens mirror natural allergens, ensuring consistency and containing key IgE epitopes.
  • Component-resolved diagnostics using recombinant allergens enable personalized allergy treatment strategies.

Purpose of the Study:

  • To review in vivo methods for assessing the biological activity of recombinant allergens.
  • To discuss current findings from in vivo testing of recombinant allergens.
  • To explore the future potential of recombinant allergens in immunotherapy.

Main Methods:

  • Description of common in vivo provocation methods: skin tests (prick, intradermal), nasal, bronchial, and conjunctival challenges.

Related Experiment Videos

  • Evaluation of biological activity of recombinant allergens through established provocation techniques.
  • Analysis of existing data from in vivo studies involving recombinant allergens.

    • Main Results:

    • Recombinant allergens can be produced with high fidelity to natural allergens, eliminating batch-to-batch variations.
    • Component-resolved diagnostics facilitate the determination of individual IgE reactivity profiles.
    • In vivo testing is essential to validate the safety and efficacy of recombinant allergens for clinical use.

    Conclusions:

    • Recombinant allergens are valuable tools for allergy diagnostics, enabling personalized treatment selection.
    • Rigorous in vivo investigation is a prerequisite for the clinical application of recombinant allergens.
    • Recombinant allergens hold significant promise for the future of allergen immunotherapy.