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Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland
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Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland

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Cryoelectron microscopy of mouse mammary tumor virus

John A G Briggs1, Barbara E Watson, Brent E Gowen

  • 1Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Headington, Oxford OX3 7BN, United Kingdom.

Journal of Virology
|February 14, 2004
PubMed

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Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...