[Study on the estrogenic effect of the organic extracts from tap water and source water]

In order to study the estrogenic effects of the organic extracts from tap water and source water, two in vitro assay and one in vivo short-term assay were performed to detect the estrogen-like activity of the component of water. The results showed that at the concentration of 50 ml relevant to real water, the recombinant yeast assay and MCF-7 cell proliferation assay could both detected the estrogen-like activity and with increase of concentration, the component exhibited an increasing estrogenic effects. The dose inducing the maximum effect in cell proliferation assay was 250 ml and in recombinant yeast assay was 1250 ml. Exceed this dose with increasing concentration, the estrogenic effects of component decreased both in two assay. In uterrtropic assay, doses of 10 L/kg and 50 L/kg increased the weight of uterus of 18 day ICR female mice significantly. All above findings indicated that organic extracts from source water exhibited the estrogenic effects. The series of 3 bioassay process displayed sensitivity and practicability.