Related Experiment Video
Updated: Jul 24, 2026

RNA Isolation from Embryonic Zebrafish and cDNA Synthesis for Gene Expression Analysis
Published on: August 7, 2009
Evidence from 18S ribosomal RNA sequences that lampreys and hagfishes form a natural group
1Department of Ecology, Ethology, and Evolution, University of Illinois, Urbana 61801.
Abstract:
Lampreys and hagfishes (cyclostomes) traditionally were considered to be a natural (monophyletic) group. Recently, the consensus of opinion, based largely on morphological analyses, has shifted to a view that lampreys are more closely related to jawed vertebrates (gnathostomes) than to hagfishes. Phylogenetic comparisons of 18S ribosomal RNA sequences from two hagfishes, two lampreys, a tunicate, a lancelet, and a number of gnathostomes support the monophyly of the cyclostomes. These data force a reassessment of several features of early vertebrate evolution.
Related Concept Videos
The Evidence for Evolution
Cis-regulatory Sequences
Evolutionary Relationships through Genome Comparisons
Multi-species Conserved Sequences
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved DNA...
FISH - Fluorescent In-situ Hybridization
RNA-seq
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

