Related Experiment Video
Updated: Aug 26, 2026
Long-Read Plasmid Sequencing and Assembly Using Nanopore Sequencing-Based Workflows
Published on: July 7, 2026
[Application of pM3, a broad host range IncP-9 plasmid, for genetic analysis in Enterobacteriaceae]
1Department of Genetics, Belarus State University, Minsk, 220050 Belarus. mtitok@bsu.by
Abstract:
The possibility of using a transposon-carrying variant of broad host range plasmid pM3 (IncP-9) as a universal vector for transposon mutagenesis and as a chromosome-mobilizing factor was demonstrated in bacteria of the family Enterobacteriaceae.
Insights
A novel plasmid vector, pM3, can be used for transposon mutagenesis and chromosome mobilization in Enterobacteriaceae bacteria. This broad host range plasmid offers a versatile tool for genetic manipulation in various bacterial species.
Area of Science:
- Microbiology
- Molecular Biology
- Bacteriology
Context:
- Broad host range plasmids are essential tools in bacterial genetics.
- Transposon mutagenesis is a key method for gene function analysis.
- The Enterobacteriaceae family includes important human pathogens and model organisms.
Purpose:
- To evaluate the potential of a transposon-carrying variant of plasmid pM3 (IncP-9) as a universal vector.
- To assess its utility as a chromosome-mobilizing factor in Enterobacteriaceae.
Summary:
- A transposon-carrying variant of the broad host range plasmid pM3 (IncP-9) was successfully utilized.
- Demonstrated its efficacy as a universal vector for transposon mutagenesis.
- Confirmed its function as a chromosome-mobilizing factor within the Enterobacteriaceae family.
Impact:
- This research introduces a versatile genetic tool for the Enterobacteriaceae.
- Facilitates advancements in bacterial genetics and functional genomics.
- Enables more efficient gene disruption and chromosomal engineering in diverse bacterial strains.
