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[Level of nitric oxide in the kidneys during apoptosis activation]

I O Komarievtseva1, O A Orlova, Ie A Blahodarenko

  • 1Lugansk State Medical University.

Ukrains'Kyi Biokhimichnyi Zhurnal (1999 )
|February 18, 2004
PubMed

Insights

Nitric oxide metabolites significantly increase in rat kidneys during apoptosis, observed in acute renal failure and compensatory hypertrophy models. This suggests peroxide processes play a role in nitric oxide

Area of Science:

  • Nephrology
  • Molecular Biology
  • Biochemistry

Context:

  • Investigated nitric oxide (NO) stable metabolites in rat kidneys under apoptosis.
  • Studied two models: acute renal failure and compensatory kidney hypertrophy post-nephrectomy.
  • Apoptosis detection utilized DNA fragmentation analysis.

Purpose:

  • To determine the relationship between nitric oxide metabolite levels and apoptosis in kidney tissue.
  • To explore the role of peroxide processes in nitric oxide's cellular effects during kidney injury and adaptation.

Summary:

  • A substantial increase in nitric oxide stable metabolites was observed in rat kidneys when apoptosis was activated.
  • Both acute renal failure and compensatory hypertrophy models showed elevated nitric oxide metabolite levels.
  • A shift in the nitrite-to-total nitrate/nitrite ratio indicated the involvement of peroxide processes.

Impact:

  • Highlights the significant role of nitric oxide metabolites in kidney apoptosis.
  • Suggests potential therapeutic targets for kidney diseases involving apoptosis and oxidative stress.
  • Provides insights into the complex interplay of nitric oxide, peroxides, and cellular fate in renal pathophysiology.

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