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DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments
Published on: January 28, 2016
Optimal enzymes for single-molecule sequencing
1Applied Molecular Evolution, Institute for Zoology, University of Leipzig, Liebigstrasse 18, D-04103 Leipzig Germany. sbrakma@rz.uni-leipzig.de
Abstract:
Much research effort has been made to realize a single-molecule sequencing. Central to this project are two enzymatic tasks that challenged several biochemists during the last years. They searched for polymerases for copying a DNA target into completely fluorophore-labeled DNA as well as for handling this new DNA derivative. Furthermore, they studied exonucleases for the sequential hydrolysis of completely dye-labeled DNA and explored optimal conditions for future application on the single-molecule level. In this article, the recent advances will be summarized from the biochemist's point of view.
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