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Seed protein variation among pepper (Capsicum sp.) genotypes revealed by MALDI-TOF analysis
Marco Aurélio Caldas de Pinho Pessoa Filho1, Carlos Bloch Junior, Danilo Fernandes da Silva Filho
1Laboratório de Citogenética e Genética Molecular, Depto de Biologia, bloco 906, Campus do Pici, Universidade Federal do Ceará, Av Humberto Monte s/n, 60.451-97, Fortaleza-CE, Brasil.
Abstract:
A method for seed proteome analysis using MALDI-TOF mass spectrometry is described. The data were used to estimate the genetic diversity degree among twelve genotypes of pepper (Capsicum). The resulting spectra were converted into a binary matrix consisting of 23 protein data sets, and genetic similarity values were calculated with the FreeTree software and Jaccard's coefficient of similarity. We have also been able to identify the presence of certain proteins in the extracts, by checking their masses on on-line databases.
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