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Fluorescent modification of N-terminal amino group in peptides for complete sequence analysis using PSD method in
Masatoshi Nakagawa1, Hiroshi Nakanishi
1Biological Information Research Center, National Institute of Advanced Industrial Science and Technology, Tsukuba Central 6, 1-1, Higashi, Tsukuba, Ibaraki, 305-8566, Japan.
Protein and Peptide Letters
|February 18, 2004
Abstract:
In the sequence analyses of peptides from nucleoprotein in influenza virus, it was very difficult to obtain the sufficient numbers of fragment ions using the post-source decay (PSD) method in MALDI-TOF-MS. Fluorescent modification of the N-terminal amino group in the target peptides was introduced for the PSD measurement. The fluorescently-labelled peptides gave sufficient fragment ions in the PSD spectrum, which leads to the complete sequence analysis of peptide.