Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Polyamine-induced actin polymerization.

C Oriol-Audit

    European Journal of Biochemistry
    |June 15, 1978
    PubMed
    Summary

    Polyamines like spermidine induce muscle actin polymerization, forming fibrous actin. This process shows a linear relationship with polyamine chain length and results in actin with properties similar to salt-induced F-actin.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    'Superpolyamines'. Macrocyclic polyamines induce highly efficient actin polymerization.

    European journal of biochemistry·1985
    Same author

    Influence of the polyamine spermine on the organization of cortical filaments in isolated cortices of Xenopus laevis eggs.

    European journal of cell biology·1985
    Same author

    Effects of polyamines on the first division cycle of Xenopus laevis eggs.

    Experimental cell research·1984
    Same author

    Supramolecular forms of actin induced by polyamines; an electron microscopic study.

    European journal of cell biology·1983
    Same author

    Actin-polyamines interaction : relationship between physicochemical properties and cytokinesis induction.

    Biochemical and biophysical research communications·1982
    Same author

    Growth of synthetic myosin filaments from myosin minifilaments.

    Biochemistry·1982

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Protein Polymerization

    Background:

    • Muscle actin exists in globular (G-actin) and fibrous (F-actin) forms.
    • Actin polymerization is crucial for muscle contraction and cellular structure.
    • Polyamines are small, positively charged molecules involved in various cellular processes.

    Purpose of the Study:

    • To investigate the effect of polyamines on muscle actin polymerization.
    • To determine the relationship between polyamine structure and actin polymerization efficiency.
    • To characterize the properties of polyamine-induced F-actin.

    Main Methods:

    • Centrifugation to quantify actin polymerization yield.
    • Spectroscopic analysis (near-ultraviolet circular dichroism) to assess protein structural changes.
    • Biochemical assays to measure Mg2+-ATPase activity.
    • Viscometry to evaluate changes in specific viscosity.
    • Electron microscopy to visualize filament formation.

    Main Results:

    • Polyamines induce reversible actin polymerization in a concentration-dependent manner.
    • Actin polymerization yield shows a linear correlation with polyamine chain length.
    • Biological polyamines, particularly spermidine and spermine, are highly efficient.
    • Polyamine-induced F-actin exhibits properties identical to salt-induced F-actin, including enhanced Mg2+-ATPase activity, increased specific viscosity, and homogeneous filament formation.

    Conclusions:

    • Polyamines are effective agents for inducing muscle actin polymerization.
    • The efficiency of polyamine-induced actin polymerization is dependent on polyamine chain length.
    • Polyamine-induced F-actin is structurally and functionally equivalent to salt-induced F-actin.

    Related Experiment Videos