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Establishment of transgenic mouse model carring the promoter of plunc sequence
Yu-fang Yang1, Yan-qing Ding, Ling Zhang
1Institute of Cancer Research, First Military Medical University, Guangzhou 510515, China. yyf@fimmu.com
Objective:
To construct the vector using specific nasopharynx tissue promoter plunc to establish transgeneic mouse model.
Methods:
Plunc-EGFP plasmid was digested with XhoI, the purified linearized DNA fragments were recovered by gel extraction and diluted to the final concentration of 4 microg/ml, before introduced into fertilized one-cell mouse eggs by pronuclear microinjection.
Results:
Thirteen founder mice were obtained, 12 of which were positive for the integrated EGFP gene as detected by PCR, and 3 were positive shown by Southern blotting.
Conclusion:
Specific nasopharynx tissue promoter plunc can effectively induce exogenous gene integration into the mouse genome.
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