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Updated: Aug 11, 2026
Robust Generation of Hepatocyte-like Cells from Human Embryonic Stem Cell Populations
Published on: October 26, 2011
Expression of nestin, an intermediate filament protein, in human fetal hepatic stem cells
1Department of Embryology and Histology, First Military Medical University, Guangzhou 510515, China.
Objective:
To explore a new oval cell marker by examining the expression of nestin in human fetal hepatic stem cells in various stages during human fetal development.
Methods:
Human fetal tissue samples of various developmental stages were obtained and fixed for preparation of paraffin sections. The expression of nestin, generally recognized as a specific marker for neural precursor cells, in fetal hepatic stem cells was detected using immunocytochemical staining (ABC method).
Results:
Nestin-positive oval cells, characterized to be hepatic stem cells, were found in the limiting plate, which arranged tightly in monolayer to form ductules that encapsulated the early-stage portal area in a sheath-like fashion, and also partially encapsulated the primary portal area. With the development of the secondary portal areas, the nestin-positive oval cells gradually centered around the Hering canal. In addition, some nestin-positive mononuclear cells were found scattering in the hepatic cords and sinusoids, especially in the mesenchymal tissue of the portal areas.
Conclusion:
Nestin-positive oval cells exist in human fetal liver and their origin and function await further investigation.
