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The use of fluorescence in situ hybridization (FISH) in chronic lymphocytic leukemia (CLL)

Alessandro Gozzetti1, Rosaria Crupi, Daniela Tozzuoli

  • 1Department of Medicine and Immunological Sciences, University of Siena, Italy. gozzetti@unisi.it

Hematology (Amsterdam, Netherlands)
|February 18, 2004
PubMed

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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...