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Developmental expression of Pop1/Bves.

Trusha K Vasavada¹, Justin R DiAngelo, Melinda K Duncan

¹Department of Biological Sciences, University of Delaware, Newark, Delaware 19716, USA.

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society

|February 18, 2004

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Pop1/Bves protein is crucial for skeletal and cardiac muscle, not coronary vessels. This study clarifies its role as a cell adhesion molecule in muscle development and regeneration.

Area of Science:

Molecular Biology
Cell Biology
Developmental Biology

Background:

Previous research suggested Pop1/Bves protein's exclusive expression in coronary smooth muscle, hinting at a role in coronary diseases.
Contradictory findings showed Pop1/Bves mRNA and activity in skeletal and cardiac muscles, with Pop1/Bves knockout mice exhibiting skeletal muscle regeneration defects.

Purpose of the Study:

To investigate the precise expression pattern and cellular localization of Pop1/Bves protein in cardiac and skeletal muscle.
To determine the functional role of Pop1/Bves in muscle development and cell adhesion.

Main Methods:

Utilized specific monoclonal antibodies (MAbs) against chicken Pop1/Bves for protein detection.
Performed immunostaining on cardiomyocytes in vitro and skeletal muscle tissue at various embryonic stages (E7, E11).

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Main Results:

Demonstrated the presence of Pop1/Bves protein in cardiomyocytes throughout development, with apparent absence in coronary vessels.
Confirmed membrane localization in cardiomyocytes involved in cell adhesion, with intracellular staining in isolated cells.
Observed Pop1 protein expression in skeletal muscle starting at embryonic day 7, with high levels in myotube cell membranes by embryonic day 11, coinciding with secondary myotube formation.

Conclusions:

Pop1/Bves protein is present in cardiomyocytes and skeletal muscle, not exclusively in coronary smooth muscle.
Pop1/Bves functions as a cell adhesion molecule, playing a role in skeletal and cardiac muscle development and potentially regeneration.