Related Experiment Video
Updated: Aug 26, 2026

Knockdown of FAM83A to Verify Its Role in Cervical Cancer Cell Growth and Cisplatin Sensitivity
Published on: February 9, 2024
[Establishment and drug-resistance gene expression of cisplatin-resistant cell line Tca8113/CDDP]
Xiao-jian Zhou1, Qin Li, Wan-tao Chen
1Department of Oral Maxillofacial Surgery, School of Stomatology, Ninth People's Hospital, Shanghai Second Medical University, Shanghai 200011, China.
Objective:
To establish a CDDP-resistant cell line from human tongue squamous cell carcinoma cell line Tca8113 and to compare the drug-resistance gene expression between them.
Methods:
The concentration of CDDP added to Tca8113 cells was increased stepwise to induce the CDDP-resistance. The index of drug-resistance was estimated by MTT assay. The expression of MDR-1, MRP, GST-pi and Bcl-2 was measured by RT-PCR optical density OD.
Results:
The CDDP-resistant cell line, Tca8113/CDDP was established, whose drug-resistance index was 9.2. The expression of MDR-1, MRP, cyclin D1 and Bcl-2 increased respectively.
Conclusion:
The expression of drug-resistance gene in Tca8113/CDDP was significantly higher than that in Tca8113.
Related Concept Videos
Treatment Resistant Cancers
Cancer-Critical Genes II: Tumor Suppressor Genes
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
Treatment Resistent Cancers
Cell Specific Gene Expression
Induced Pluripotent Stem Cells
Somatic cells are...