Related Experiment Video
Updated: Aug 26, 2026
Formation of Human Periodontal Ligament Cell Spheroids on Chitosan Films
Published on: June 19, 2019
[Cell behavior of periodontal ligament cells cultured on commercially pure titanium]
Bin Zhou1, Xiang-rong Cheng, Tao Jiang
1Department of Stomatology, Tongji Hospital, Huazhong University of Science and Technology, Wuhan 430030, China.
Objective:
To study the cell behavior of periodontal ligament cells (PDLCs) cultured on commercially pure titanium (cpTi) by cell morphology, cell counting and osteoclin (OC) immunofluorescent staining.
Methods:
1 x 10(5)/ml PDLCs in 2 ml culture medium were seeded on cpTi discs fixed in 24-well culture plates. Morphological study of cell attachment was observed by scanning electron microscope (SEM) and fluorescence microscopy. Cell adhesion was analyzed by MTT at 0.5, 1, 2, 4 hours respectively. OC was directly observed by immunofluorescent staining.
Results:
PDLCs could attach and spread on cpTi discs. SEM showed that PDLCs had pseudopod-like protuberance. PDLCs showed different attaching phase and reached saturation in cell number at the early stage. The cells and ECM could express OC 7 days later.
Conclusion:
PDLCs show a regular, dynamic pattern in early stage and they can keep proliferating and differentiating on CpTi in vitro. It was indicated that the biological function of PDLCs around dental implant should be reconsidered.
