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[Determination of luteolin and luteolin-7-beta-D-glucoside in Chrysanthemum morfolium Ramat. from different

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Summary

The study observed luteolin and luteolin-7-beta-D-glucoside content in Chrysanthemum morifolium Ramat. (CMR) over time. Luteolin-7-beta-D-glucoside levels varied with collection time, indicating its potential as a quality indicator for CMR.

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Area of Science:

  • Phytochemistry
  • Pharmacognosy
  • Analytical Chemistry

Background:

  • Chrysanthemum morifolium Ramat. (CMR) is a traditional medicinal herb.
  • Luteolin and luteolin-7-beta-D-glucoside are key bioactive compounds in CMR.
  • Understanding their content variation is crucial for quality control.

Purpose of the Study:

  • To investigate the quantitative changes of luteolin and luteolin-7-beta-D-glucoside in CMR.
  • To determine the impact of collection time on the content of these two compounds.
  • To assess the suitability of these compounds as quality markers for CMR.

Main Methods:

  • Samples of Chrysanthemum morifolium Ramat. were collected in 2001 and 2002.
  • Reverse-phase high-performance liquid chromatography (RP-HPLC) was employed for analysis.
  • Quantification of luteolin and luteolin-7-beta-D-glucoside was performed.

Main Results:

  • Luteolin-7-beta-D-glucoside content was significantly higher than luteolin content.
  • Luteolin content remained relatively stable across different collection times.
  • Luteolin-7-beta-D-glucoside content showed minimal variation in early collection but decreased significantly in later collection periods.

Conclusions:

  • Luteolin-7-beta-D-glucoside is a more reliable indicator of Chrysanthemum morifolium Ramat. quality than luteolin.
  • Collection time significantly influences the content of luteolin-7-beta-D-glucoside in CMR.
  • These findings support the use of luteolin-7-beta-D-glucoside for quality assessment of CMR.