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Updated: Aug 4, 2026
High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
Published on: April 23, 2019
[HPLC determination of zolmitriptan and its related substances]
Yun-zhen Hu1, Tong-wei Yao, Xiang-jun Wang
1College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310031 China.
Objective:
To develop an analytical method and quality control for determination of zolmitriptan and related substances.
Methods:
Zolmitriptan and related substances were separated and determined on a shimadzu CLC-C(8) column (150 mm x 6 mm, 10 microm) with a mobile phase of acetonitrile-10 mmol/L phosphate buffer (25:75 pH 7.5) and a flow-rate of 1 ml/min; the UV-VIS detector was operated at 229 nm.
Result:
The limit of detection for the related substances was 0.5 ng on the zolmitriptan basis (S/N >3). Linear calibration curve was gene rated from 4 - 40 microg/ml with a correlation coefficient of 0.9999. The recovery rate of zolmitriptan was 99.1% with a standard deviation of 0.2%. The results of HPLC method were consistent with those of nonaqueous titration method.
Conclusion:
HPLC method is a rapid sensitive and accurate method for the determination of zolmitriptan and its related substances.
