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Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells
12:16

Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells

Published on: December 17, 2015

[Cloning and expression of Porphyromonas gingivalis pgmA gene encoding outer membrane protein associated with

Chun-yan Zhuang1, Li-li Chen, Jie Yan

  • 1Department of Stomatology, The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009 China.

Zhejiang Da Xue Xue Bao. Yi Xue Ban = Journal of Zhejiang University. Medical Sciences
|February 18, 2004
PubMed
Abstract

Insights

Researchers successfully cloned the Porphyromonas gingivalis pgmA gene and developed an expression system for its fusion protein. This protein demonstrated significant immunogenicity and immunoreactivity, making it a promising candidate for P. gingivalis detection and vaccine development.

Area of Science:

  • Molecular Biology
  • Microbiology
  • Immunology

Context:

  • Porphyromonas gingivalis is a key pathogen in periodontitis.
  • The pgmA gene's role in P. gingivalis pathogenesis is of significant interest.
  • Developing diagnostic tools and vaccines against P. gingivalis requires understanding its antigens.

Purpose:

  • To clone the pgmA gene from Porphyromonas gingivalis.
  • To construct an expression vector for the pgmA gene.
  • To identify the immunogenicity and immunoreactivity of the resulting fusion protein.

Summary:

  • The pgmA gene was successfully amplified and sequenced from two P. gingivalis strains, showing high sequence homology.
  • A pET32a expression vector containing the pgmA gene was constructed, leading to high-level expression of a PgmA fusion protein in E. coli.
  • The PgmA fusion protein induced specific antibodies in rabbits and reacted with a high percentage (92.3%) of P. gingivalis isolates.

Impact:

  • Established a highly efficient expression system for the P. gingivalis pgmA gene.
  • The PgmA fusion protein exhibits satisfactory immunogenicity and immunoreactivity.
  • The PgmA fusion protein is a potential candidate antigen for P. gingivalis detection and vaccine development.

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