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Related Experiment Videos

A novel method for "Wet" SEM.

Iris Barshack1, Juri Kopolovic, Yehuda Chowers

  • 1Department of Pathology, The Chain Sheba Medical Center, Tel-Hashomer, Affiliated to the Tel-Aviv University, Sackler School of Medicine, Israel. barshack@sheba.health.gov.il

Ultrastructural Pathology
|February 18, 2004
PubMed
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Wet Scanning Electron Microscopy (Wet SEM) allows imaging of hydrated tissues without fixation. This advancement simplifies microscopic examination for clinical diagnosis and research applications.

Area of Science:

  • Microscopy
  • Materials Science
  • Cell Biology

Background:

  • Microscopic examination of tissues is crucial for clinical diagnosis and research.
  • Traditional methods require complex fixation and preparation procedures for wet tissues.
  • Advancements in imaging techniques can streamline tissue analysis.

Purpose of the Study:

  • To present the application of Wet Scanning Electron Microscopy (Wet SEM) for imaging fully hydrated biological samples.
  • To demonstrate a method for examining wet tissues without fixation or complex preparation.
  • To highlight the potential of Wet SEM in simplifying microscopic analysis.

Main Methods:

  • Utilizing a specialized Wet SEM technology capable of imaging samples at atmospheric pressure.
  • Employing sealed specimen capsules with a thin, electron-transparent partition membrane to maintain a 1-atm pressure difference.

Related Experiment Videos

  • Performing imaging in a scanning electron microscope (SEM) using backscattered electron detection.

    • Main Results:

    • Successfully imaged fully hydrated samples at atmospheric pressure using Wet SEM.
    • Demonstrated the capability of the partition membrane to withstand a 1-atm pressure difference.
    • Obtained cellular-level information through backscattered electron detection penetrating the specimen.

    Conclusions:

    • Wet SEM offers a simplified approach to microscopic examination of wet tissues.
    • The technique eliminates the need for fixation and complex preparation, facilitating broader application.
    • Wet SEM holds significant potential for clinical diagnosis and diverse research fields.