Related Experiment Video
Updated: Aug 26, 2026

Air-Inflation of Murine Lungs with Vascular Perfusion-Fixation
Published on: February 2, 2021
Lung fixation for the preservation of air spaces
Peter Blümler1, Rodolfo H Acosta, Angelika Thomas-Semm
1Max-Planck Institut für Polymerforschung, Mainz, Germany.
Abstract:
The procedures for the fixation of entire lungs of small rodents are presented together with various techniques used to verify the structural integrity of the lung tissue. To achieve this, the lungs were dissected out from rats and mice killed by ether overdose. The specimens were rinsed with isotonic saline and fixation solution under low vacuum conditions. After fixation, they were dried using alcohol and stored in a noncollapsed state (i.e., state of inhalation). Light and scanning electron microscopy as well as magnetic resonance imaging using hyperpolarized 3He were employed to verify the intact state on interalveolar septa and walls of smaller bronchi as well as accessibility of the air spaces.
Related Concept Videos
Cardiopulmonary Resuscitation II: ACLS Airway Management
Flail Chest-II
Assessment:
1. Clinical Evaluation:
History:
Cardiopulmonary Resuscitation V: Advanced Airway Management Techniques
Pneumothorax-II
Clinical Manifestations:
Pleura of the Lungs
Oxygen Delivering System II: Venturi Mask and Transtracheal Oxygen
Venturi Mask
The Venturi mask, named after the Venturi effect, is designed to deliver precise oxygen concentrations. It consists of a large tube with an oxygen inlet that narrows down, causing a pressure drop that pulls air in through adjustable side ports. The mask is a lightweight,...

