Effect of intestinal content contamination on broiler carcass Campylobacter counts

Intestinal contents may contaminate broiler carcasses during processing. The objective of this study was to determine what effect various levels of intestinal contents had on the numbers of Campylobacter detected in broiler carcass rinse samples. Eviscerated broiler carcasses were collected from the shackle line in a commercial processing plant immediately after passing through an inside/outside washer. Broiler carcasses were cut longitudinally into contralateral halves using a sanitized saw. Cecal contents from the same flock were collected, pooled, homogenized, and used to contaminate carcass halves. Paired carcass halves were divided into groups of eight each, and then cecal contents (2, 5, 10, 50, or 100 mg) were placed onto one randomly selected half of each carcass, while the corresponding half of the same broiler carcass received no cecal contents. Campylobacter counts from carcass halves with cecal contamination were compared to the uncontaminated halves of the same carcasses using a paired t test. Carcass halves with 5 mg or more of surface cecal contamination had significantly higher numbers of Campylobacter than those without (P < 0.01). Carcass halves contaminated with only 5 mg of cecal contents had an average of 3.3 log CFU Campylobacter per ml of rinse, while corresponding uncontaminated carcass halves had 2.6 log CFU Campylobacter per ml of rinse. These data indicate that even small (5 mg) amounts of cecal contents can cause a significant increase in the numbers of Campylobacter on eviscerated broiler carcasses. Therefore, it is important to keep such contamination to a minimum during processing.