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Updated: Aug 26, 2026
An Efficient and Reproducible Method for Producing Composite Plants by Agrobacterium rhizogenes-Based Hairy Root Transformation
Published on: June 30, 2023
Agrobacterium rhizogenes-mediated transformation to improve rooting ability of eucalypts
1FRD/UN Research Unit for Plant Growth and Development, Department of Botany, University of Natal, P.O. Box 375, Pietermaritzburg 3200, South Africa.
Abstract:
In an attempt to improve rooting in in vitro propagated Eucalyptus clones, root-inducing genes on the Ri plasmids of three Agrobacterium rhizogenes strains were inserted into the Eucalyptus genome. Root development was monitored in vitro and after the plantlets had hardened off. Only transformed roots grew as root cultures in hormone-free liquid medium. The potential use of this procedure for improving rooting of clonal material is discussed.
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