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Site-specific thromboembolism: a novel animal model for stroke
Andrew J Ringer1, Lee R Guterman, L Nelson Hopkins
1Department of Neurosurgery and Toshiba Stroke Research Center, School of Medicine and Biomedical Sciences, University at Buffalo, State University at New York, Buffalo, NY, USA.
AJNR. American Journal of Neuroradiology
|February 19, 2004
Summary
This study introduces a reliable animal model for creating site-specific thrombi. This technique is crucial for accurately evaluating intravascular thrombolysis and clot retrieval methods.
Area of Science:
- Biomedical Engineering
- Vascular Surgery
- Interventional Radiology
Background:
- Developing effective treatments for thrombotic events requires accurate preclinical models.
- Current models may lack precision in thrombus placement and volume control.
Purpose of the Study:
- To establish a reproducible technique for site-specific thrombus creation in an animal model.
- To facilitate the evaluation of intravascular thrombolysis and clot retrieval strategies.
Main Methods:
- Six swine underwent bilateral ascending pharyngeal artery (APA) thrombus injection.
- A balloon catheter and microcatheter system was used for precise thrombus delivery.
- Thrombus composed of autologous blood and bovine thrombin was injected during temporary flow arrest.
Main Results:
- Angiography confirmed successful APA occlusion without recanalization over 3 hours.
- Gross and histologic examination revealed organized thrombus without intimal injury.
- Thrombus placement was confined to the proximal APA.
Conclusions:
- The developed model offers a simple and reliable method for site-specific thrombus injection.
- This technique allows for predetermined thrombus volume and localization, essential for efficacy studies.
- The model is well-suited for comparing thrombolytic agents and endovascular techniques.