Related Experiment Videos
Adenovirus-mediated gene delivery: an overview
1Division of Human Gene Therapy, Departments of Medicine, Pathology, and Surgery and the Gene Therapy Center, University of Alabama at Birmingham, Birmingham, AL, USA.
Methods in Molecular Biology (Clifton, N.J.)
|February 19, 2004
Summary
Adenoviruses, discovered in the 1950s, are widely used as gene-delivery vectors. These viruses are classified into genera, subgroups, and over 50 serotypes based on genetic and agglutination properties.
Area of Science:
- Virology
- Molecular Biology
- Gene Therapy
Background:
- Adenoviruses, family Adenoviridae, were first isolated in the 1950s.
- Since the early 1980s, adenoviruses have been developed as gene-delivery vehicles (vectors).
- The Adenoviridae family includes Aviadenovirus (avian hosts) and Mastadenovirus (mammalian hosts) genera.
Purpose of the Study:
- To provide an overview of adenovirus classification and applications.
- To detail the categorization of human adenoviruses.
- To highlight the historical development of adenoviruses as gene-delivery vectors.
Main Methods:
- Classification based on guanine-cytosine content and red blood cell agglutination.
- Subdivision into six subgroups.
- Serotype determination using neutralization assays.
Main Results:
- Human adenoviruses are classified into six subgroups.
- Over 50 distinct serotypes have been identified.
- Adenoviruses serve as versatile gene-delivery vectors.
Conclusions:
- Adenovirus classification relies on genetic and serological methods.
- The diverse serotypes of adenoviruses offer potential for various applications.
- Adenoviruses are significant tools in gene therapy and molecular biology research.