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Monitoring changes in the subcellular location of proteins in S. cerevisiae

Diego Rua1, Teresa Holzen, Benjamin S Glick

  • 1Center for Molecular Oncology, University of Chicago, IL, USA.

Methods in Molecular Biology (Clifton, N.J.)
|February 19, 2004
PubMed
Abstract

No abstract available in PubMed .

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Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...