Related Experiment Video
Updated: Jun 25, 2026

RNA-Seq Analysis of Differential Gene Expression in Electroporated Chick Embryonic Spinal Cord
Published on: November 1, 2014
Sequence analysis of a rainbow trout cDNA library and creation of a gene index
C E Rexroad1, Y Lee, J W Keele
1USDA/ARS National Center for Cool and Cold Water Aquaculture, Kearneysville, West Virginia 25430, USA. crexroad@ncccwa.ars.usda.gov
Abstract:
Expressed sequence tag (EST) projects have produced extremely valuable resources for identifying genes affecting phenotypes of interest. A large-scale EST sequencing project for rainbow trout was initiated to identify and functionally annotate as many unique transcripts as possible. Over 45,000 5' ESTs were obtained by sequencing clones from a single normalized library constructed using mRNA from six tissues. The production of this sequence data and creation of a rainbow trout Gene Index eliminating redundancy and providing annotation for these sequences will facilitate research in this species.

