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Polypeptides associated with Pasteurella multocida infection in rabbits.

T E Zimmerman1, B J Deeb, R F DiGiacomo

  • 1Department of Comparative Medicine, School of Medicine, University of Washington, Seattle 98195.

American Journal of Veterinary Research
|July 1, 1992
PubMed
Summary

Researchers identified three key Pasteurella multocida polypeptides (28, 30, and 37 kDa) that are consistently detected by antibodies after infection. These findings suggest potential for improved serologic detection of Pasteurella multocida in rabbits.

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Detection of Antibodies to Pasteurella multocida by capture enzyme immunoassay using a monoclonal antibody against P37 antigen.

Journal of clinical microbiology·1997

Area of Science:

  • Veterinary Microbiology
  • Immunology
  • Bacteriology

Background:

  • Pasteurella multocida causes significant disease in rabbits.
  • Accurate serologic detection methods are crucial for managing Pasteurella multocida infections.

Purpose of the Study:

  • To identify specific Pasteurella multocida antigens recognized by rabbit antibodies during infection.
  • To evaluate the potential of these antigens for developing diagnostic tools.

Main Methods:

  • Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was used to separate P. multocida polypeptides.
  • Immunoblot analysis with rabbit sera was performed to detect antigen-antibody reactions.
  • Sera were collected from experimentally infected and naturally infected rabbits.

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Main Results:

  • Three P. multocida polypeptides (approx. 28, 30, and 37 kDa) were consistently recognized by antibodies post-infection.
  • These same polypeptides were detected in both P. multocida serotype A:12 and A:3 antigens.
  • Antibodies from naturally infected rabbits also reacted with these specific polypeptides.

Conclusions:

  • The identified 28, 30, and 37 kDa polypeptides are strong candidates for serologic detection of Pasteurella multocida.
  • These antigens may facilitate the development of more sensitive and specific diagnostic assays for P. multocida infections in rabbits.