Related Experiment Video
Updated: Aug 26, 2026

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1
Published on: May 27, 2016
Porcine adenovirus type 3 E1 transcriptional control region contains a bifunctional regulatory element
1Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, Saskatchewan, Canada S7N 5E3.
Abstract:
We identified a bifunctional regulatory element located between nt 374 and 431 upstream of TATA box of porcine adenovirus (PAV) 3 E1A promoter. Deletion of the element dramatically reduced the steady-state level of E1A mRNA, but increased that of E1B, which lies immediately downstream of E1A. The mutant virus displayed defective replication at early times of infection, but replicated nearly as efficiently as wild-type PAV-3 at late times of infection. This defect was complemented with coinfecting wild-type virus in a mixed infection. The results indicated that the upstream activation sequences (UAS) of E1A overlap the upstream repression sequences (URS) of E1B, although both transcription units are transcribed from different promoters.
Related Concept Videos
RNA Polymerase II Accessory Proteins
Master Transcription Regulators
Master Transcription Regulators
The Eukaryotic Promoter Region
The Eukaryotic Promoter Region
Prokaryotic Transcriptional Activators and Repressors
Transcription of prokaryotic...
