Axonal misdirection as contributing factor to aberrant reinnervation of muscles after facial nerve suture in cats

Abstract Whereas basic features of post-axotomy muscle reinnervation have been extensively studied in rats, little is known about axonal regrowth and pathfinding in cats. To address the question, adult cats were subjected to facial-facial anastomosis (FFA). First group served to establish optimal parameters for labeling of the zygomatic and buccal facial branches with 1,1'dioctadecyl-3,3,3,'3'-tetramethylindo-carbocyanine perchlorate (DiI) and Fast Blue (FB) placed onto respective transected nerves. The second group of animals underwent identical bilateral labeling 3 months after transection and suture of the right facial nerve. This group served to establish the number of motoneurons, which had branched after surgery and projected into both facial branches. On control side, DiI application onto zygomatico-orbital branch labeled 3883 +/- 598 (mean +/- S.D.) perikarya were confined to the dorsal and intermediate facial subnuclei, meanwhile an application of FB onto the buccal branch labeled 1617 +/- 552 perikarya in the lateral and ventrolateral subnuclei. There were no double-labeled cells. Three months after FFA all retrogradely labeled motoneurons were scattered throughout the entire facial nucleus. To establish the proportion of perikarya, that re-grew multiple axonal branches into both nerves, double-labeled (FB + DiI) motoneurons were counted from digital images. The zygomatico-orbital nerve contained 3311 +/- 430 DiI-labeled whereas the buccal nerve 1500 +/- 442 FB-labeled motoneurons. The occurrence of 311 +/- 103 double-labeled perikarya (DiI+FB) suggested that approximately 6% of all retrogradely labeled motoneurons branched axons into both nerves. I conclude that malfunctioning axonal pathfinding rather than deviant reinnervation contributed to poor recovery of function after FFA in the cat.