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Updated: Aug 26, 2026

04:53
Detection of Total Reactive Oxygen Species in Adherent Cells by 2',7'-Dichlorodihydrofluorescein Diacetate Staining
Published on: June 23, 2020
A ratiometric fluorescent probe for imaging hydroxyl radicals in living cells
Nobuaki Soh1, Koji Makihara, Emino Sakoda
1Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
Abstract:
A novel fluorescent probe, the detection mechanism of which is based on the 'on-off' switching of a FRET triggered by the *OH-induced cleavage of a DNA strand, has been developed for the ratiometric imaging of *OH.

