Calpain 10 gene polymorphisms and the risk of ischaemic stroke in a Polish population

Experimental studies showed that calpain inhibitors suppressed post-ischaemic changes in the brain. We examined whether an association exists between variants of three single nucleotide polymorphisms (SNPs) -43, -19, and -63 in the calpain 10 gene and either stroke or size of ischaemic lesions on the CT in a Polish population. We included 209 patients with a first ischaemic stroke and 148 controls. The patients were divided into four groups based on the infarct size assessed on the CT taken within two week after stroke. Alleles of SNP19 were determined by electrophoresis of the PCR product on agarose gel by size; while for SNP43 and -63 the RFLP method was used. The allele frequencies for patients and controls were similar in both groups: SNP43- G/A- 73.5%/26.5% vs. 71.0%/29%, SNP19-three 32 bp/two 32 bp repeats--65.7%/34.2% vs. 62.2%/37.7%; SNP63- C/T- 89.5%/10.5% vs. 90.9%/9.1%, respectively. The distribution of the genotypes, haplotypes, and haplotype combinations did not differ in both groups. The distribution in the subgroups of patients based on the size of the ischaemic lesions was similar to controls. Our study did not showed any association between calpain 10 SNPs: -43, -19, and -63 and ischaemic stroke, or with the size of post-ischaemic cerebral lesions in a Polish population.