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Published on: December 28, 2016
A hypothermic-temperature-sensitive gene silencing by the mammalian RNAi
Takashi Kameda1, Kenji Ikegami, Yang Liu
1Department of Biochemistry, Akita University School of Medicine 1-1-1 Hondo, Akita 010-8543, Japan.
Abstract:
RNA interference (RNAi) has been attracting a great deal of attention. This pathway is highly conserved among most eukaryotes and believed to be important for antiviral reactions and epigenetic gene regulation. Because a temperature-sensitive RNAi was reported in both plant and insect systems, suggesting its evolutional conservation, we analyzed the effect of different temperatures on mammalian RNAi, targeting the ectopic gene expression, and detected suppression at hypothermic temperatures. This phenomenon could be critical and useful to control ectopic and internal gene expressions by RNAi.
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