Related Experiment Videos
Uptake of Al3+ into the N-lobe of human serum transferrin
G Kubal1, A B Mason, P J Sadler
1Department of Chemistry, Birkbeck College, University of London, U.K.
The Biochemical Journal
|August 1, 1992
Abstract:
We have studied the binding of Al3+ to human serum apotransferrin (80 kDa) and recombinant N-lobe human apotransferrin (40 kDa) in 0.1 M-sodium bicarbonate solution at a pH meter reading in 2H2O (pH*) of 8.8 using 1H n.m.r. spectroscopy. The results show that for the intact protein, preferential binding of Al3+ to the N-lobe occurs. Molecular modelling combined with an analysis of ring-current-induced shifts suggest that n.m.r. spectroscopy can be used to probe hinge bending processes which accompany metal uptake in solution.