Related Experiment Video
Updated: Aug 26, 2026
Treatment of Osteochondral Defects in the Rabbit's Knee Joint by Implantation of Allogeneic Mesenchymal Stem Cells in Fibrin Clots
Published on: May 21, 2013
Biomechanical and histological evaluation of osteochondral transplantation in a rabbit model
Ellis K Nam1, Mohsen Makhsous, Jason Koh
1Department of Orthopaedic Surgery, Northwestern Medical School, Chicago, Illinois, USA. ellisnam@aol.com
Background:
Biomechanical and histological properties of osteochondral transplantation have not been extensively examined.
Hypothesis:
Osteochondral grafts have properties similar to native articular cartilage.
Study Design:
Controlled laboratory study.
Methods:
A 2.7 mm (diameter) x 4.0 mm (depth) osteochondral defect was created in 17 New Zealand white rabbit knees. An osteochondral graft, harvested from the contralateral knee, was transplanted into the defect. Eight rabbits were sacrificed each at 6 and 8 weeks.
Results:
The 12-week grafts (1213.6 +/- 309.0 N/mm) had significantly higher stiffness than the 6-week grafts (483.1 +/- 229.1 N/mm; P <.001) and of normal cartilage (774.8 +/- 117.1 N/mm; P <.003). Stiffness of the 6-week grafts was significantly lower than normal cartilage (P <.036). At all time points, full-thickness defects had significantly lower stiffness than normal cartilage (P <.001). Histologically, transplanted grafts scored significantly higher than the full-thickness defects (P <.001). The defects showed inconsistent, fibrocartilage healing. The grafts demonstrated cartilage viability, yet with a persistent cleft between the graft and host.
Conclusions:
Osteochondral transplants undergo increased stiffness in the short term, with evidence of structurally intact grafts.
Clinical Relevance:
Osteochondral transplantation may be a viable treatment option; however, long-term investigation on graft function is necessary.
