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Updated: Aug 26, 2026
A Model of Acute Lung Injury Following Visceral Ischemia-Reperfusion by Supra-Coeliac Aortic Cross Clamping in Rats
Published on: August 15, 2025
Lung perfusion with oxygenated blood during aortic clamping prevents lung injury
Jing-Hao Zheng1, Zhi-Wei Xu, Wei Wang
1Department of Pediatric Thoracic and Cardiovascular Surgery, Xin Hua Hospital, Shanghai Children's Medical Center and Shanghai Second Medical University Shanghai, Pudong, People's Republic of China. zhjh@sh136a.sta.net.cn
Insights
Continuous pulmonary perfusion with oxygenated blood protected lung function during cardiopulmonary bypass. This technique prevented lung injury, preserving pulmonary parenchyma and reducing edema and neutrophil infiltration.
Area of Science:
- Cardiovascular Surgery
- Pulmonary Medicine
- Biomedical Engineering
Background:
- Aortic crossclamping during cardiopulmonary bypass can lead to lung injury.
- Pulmonary anoxia is a significant factor in this injury.
- Protective strategies are needed to mitigate lung damage.
Purpose of the Study:
- To evaluate the protective effect of continuous pulmonary perfusion with oxygenated blood.
- To assess the impact of this technique on pulmonary function and histology.
- To determine if continuous perfusion prevents lung injury during aortic crossclamping.
Main Methods:
- 12 piglets underwent cardiopulmonary bypass for 130 minutes.
- An experimental group received continuous pulmonary perfusion with oxygenated blood.
- A control group did not receive continuous perfusion.
- Pulmonary function and histology were assessed before and after bypass.
Main Results:
- Pulmonary function was significantly better in the experimental group post-bypass.
- The experimental group showed preserved pulmonary parenchyma.
- The control group exhibited marked intra-alveolar edema and neutrophil infiltration.
Conclusions:
- Continuous pulmonary perfusion with oxygenated blood is effective in preventing lung injury.
- Pulmonary anoxia during aortic crossclamping is a primary cause of lung damage.
- This perfusion strategy offers a protective effect on lung tissue.
Abstract:
To evaluate the protective effect of continuous pulmonary perfusion with oxygenated blood during aortic crossclamping, 12 mixed-breed piglets (7-12 kg) were placed on cardiopulmonary bypass for 130 minutes. An experiment group of 6 (group E) had continuous pulmonary perfusion with oxygenated blood during cardiopulmonary bypass, while the other 6 served as controls (group C). Pulmonary function was measured at the beginning and end of cardiopulmonary bypass and one hour later. Histology was compared before and after cardiopulmonary bypass. Pulmonary function after cardiopulmonary bypass was significantly better in group E than group C. There was preservation of the normal pulmonary parenchyma in group E, whereas group C had marked intra-alveolar edema and abundant intra-alveolar neutrophils. Anoxia of lung tissue during aortic crossclamping on cardiopulmonary bypass is probably the major factor in lung injury. Continuous pulmonary perfusion was effective in preventing lung injury during aortic crossclamping.
