Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Genotyping using single nucleotide polymorphism, fluorescence spectroscopy and pattern recognition.

Richard G Brereton1, Martin Devonshire

  • 1School of Chemistry, University of Bristol, Cantock's Close, Bristol BS8 1TS, UK.

The Analyst
|February 24, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tapping the Full Potential of Infrared Spectroscopy for the Analysis of Technical Lignins.

ChemSusChem·2024
Same author

Evaluation of cataract formation in fish exposed to environmental radiation at Chernobyl and Fukushima.

The Science of the total environment·2023
Same author

Chemometrics in analytical chemistry-part II: modeling, validation, and applications.

Analytical and bioanalytical chemistry·2018
Same author

High-density functional-RNA arrays as a versatile platform for studying RNA-based interactions.

Nucleic acids research·2018
Same author

Chemometrics in analytical chemistry-part I: history, experimental design and data analysis tools.

Analytical and bioanalytical chemistry·2017
Same author

European Analytical Column No. 42.

Analytical and bioanalytical chemistry·2014
Same journal

A two-step centrifugal microfluidic platform for semi-automated IGRA detection of tuberculosis based on chemiluminescence.

The Analyst·2026
Same journal

On-site rapid identification of animal and plant creams <i>via</i> 2D FeB nanozyme-based colorimetric sensors.

The Analyst·2026
Same journal

Sensitive detection of aflatoxin B1 using a dual-mode fluorescent aptasensor based on cascade signal amplification.

The Analyst·2026
Same journal

Deep learning-enabled microfluidic digital PCR platform for efficient seven-color quantification.

The Analyst·2026
Same journal

Monitoring food spoilage biogenic amines utilizing a blue-emitting fluorescent ionic liquid.

The Analyst·2026
Same journal

Correction: Regeneration-on-a-chip: a planarian microfluidic device enabling automated cultivation, individual tracking and <i>in vivo</i> imaging for regeneration study.

The Analyst·2026
See all related articles

This study presents a novel genetic screening method using single nucleotide polymorphism (SNP) analysis. The technique accurately classifies human DNA samples into wild type, mutant, and mixed alleles for genetic research.

Area of Science:

  • Genetics
  • Molecular Biology
  • Bioinformatics

Background:

  • Genetic screening is crucial for identifying disease-associated mutations.
  • Single nucleotide polymorphisms (SNPs) are key genetic markers.
  • Accurate classification of alleles (wild type, mutant, mixed) is essential for genetic studies.

Purpose of the Study:

  • To develop and validate a novel method for genetic screening.
  • To accurately differentiate between wild type, mutant, and mixed alleles using fluorescence spectroscopy and principal component analysis.
  • To enable efficient and reliable genetic analysis.

Main Methods:

  • Human DNA fragments were analyzed using fluorescence spectroscopy with specific fluorescent probe dyes.
  • Taqman reaction was employed for sample preparation.

Related Experiment Videos

  • Principal component analysis (PCA) was used to process fluorescence spectra.
  • Malahalobis distance with 4 principal components was applied for sample classification.

    • Main Results:

    • Fluorescence spectra were recorded for human DNA fragments.
    • PCA successfully separated spectra into three distinct groups corresponding to allele 1 (wild type), allele 2 (mutant), and mixed allele.
    • Malahalobis distance achieved correct classification of samples into these groups.

    Conclusions:

    • The described method provides an effective approach for genetic screening.
    • The combination of fluorescence spectroscopy, PCA, and Malahalobis distance enables accurate SNP genotyping.
    • This method facilitates reliable identification of genetic variations in human DNA.