Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Toolbox for retrovectorologists.

J C Pagès1, Thierry Bru

  • 1Vector Group, D3M, Faculté de Médecine, 2 bd Tonnellé, 37000 Tours, France. pages@med.univ-tours.fr

The Journal of Gene Medicine
|February 24, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evoked response activity eigenmode analysis in a convoluted cortex via neural field theory.

Physical review. E·2021
Same author

Neural field theory of perceptual echo and implications for estimating brain connectivity.

Physical review. E·2018
Same author

Immune-Complexed Adenovirus Induce AIM2-Mediated Pyroptosis in Human Dendritic Cells.

PLoS pathogens·2016
Same author

Predictors of disease-free survival in colorectal cancer with microsatellite instability: An AGEO multicentre study.

European journal of cancer (Oxford, England : 1990)·2015
Same author

Effect of low-frequency KRAS mutations on the response to anti-EGFR therapy in metastatic colorectal cancer.

Annals of oncology : official journal of the European Society for Medical Oncology·2013
Same author

A Siglec-like sialic-acid-binding motif revealed in an adenovirus capsid protein.

Glycobiology·2012
Same journal

Identification and Experimental Verification of Key Genes Related to the Ras Signaling Pathway and the Hippo Signaling Pathway in Osteoarthritis Based on Transcriptome Data.

The journal of gene medicine·2026
Same journal

ZNF468 Exhibits Oncogenic Activity by Regulating Unexplored ZNF707 in Breast Cancer.

The journal of gene medicine·2026
Same journal

The Histidine-Rich Glycoprotein A1043G Polymorphism and Susceptibility to Recurrent Miscarriage in Erbil-The Kurdistan Region of Iraq.

The journal of gene medicine·2026
Same journal

In Vivo Assessment of dbDNA GNE<sup>wt</sup>/bi-shRNA-GNE<sup>M743T</sup> Lipoplex for GNE Myopathy: Improved Potency and Safety.

The journal of gene medicine·2026
Same journal

F2R Promotes Prostate Cancer Progression via COL8A1-Dependent Activation of the FAK/PI3K/AKT Signaling Axis.

The journal of gene medicine·2026
Same journal

Rational Design and Optimisation of CRISPR-Cas9 Delivery Systems for Targeted Genomic Transformation.

The journal of gene medicine·2026
See all related articles

Gene therapy using retroviral vectors shows promise but faces challenges in cell transduction. Further research into the retroviral life cycle is crucial for improving vector efficacy and safety in therapeutic applications.

Area of Science:

  • Molecular Biology
  • Gene Therapy
  • Virology

Background:

  • Retroviral vectors are key tools in gene therapy, but challenges like target resistance to transduction have emerged.
  • Despite early successes and the development of lentiviral vectors, a deeper understanding of the retroviral life cycle is needed.
  • Recent developments indicate ongoing challenges in adapting vectors for specific cell targeting.

Purpose of the Study:

  • To review key features of the wild-type retroviral life cycle.
  • To provide an overview of the requirements for generating retroviral vectors.
  • To discuss recent developments and critical biosafety issues in retroviral vector technology.

Main Methods:

  • Review of existing literature on retroviral life cycle and vector generation.

Related Experiment Videos

  • Analysis of advancements in retroviral vector development.
  • Highlighting critical biosafety considerations.

    • Main Results:

    • Retroviral vector use in gene therapy has advanced significantly.
    • Challenges in transduction efficiency and target specificity persist.
    • Biosafety remains a paramount concern in retroviral vector applications.

    Conclusions:

    • Continued research into the retroviral life cycle is essential for overcoming transduction barriers.
    • Further optimization of retroviral vectors is required for safe and effective gene therapy.
    • Addressing biosafety issues is critical for the clinical translation of gene therapy.