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Surface-engineering of lentiviral vectors
Els Verhoeyen1, François-Loïc Cosset
1Laboratoire de Vectorologie Rétrovirale et Thérapie Génique, INSERM U412, Ecole Normale Supérieure de Lyon, IFR128 BioSciences Lyon-Gerland. Lyon, France.
The Journal of Gene Medicine
|February 24, 2004
Summary
Lentiviral vectors offer flexible gene transfer but struggle with non-proliferating cells. Innovative approaches are being explored to enhance lentiviral vector capabilities for effective in vivo gene delivery to diverse tissues.
Area of Science:
- Gene Therapy
- Molecular Virology
- Biotechnology
Background:
- Retroviral vectors provide adaptable gene transfer through glycoprotein-core associations.
- Lentiviral vectors are superior to onco-retroviral vectors (e.g., MLVs) as they can transduce non-proliferating cells.
Purpose of the Study:
- To review innovative strategies for upgrading lentiviral vectors.
- To develop lentiviral vectors suitable for in vivo gene delivery applications.
Main Methods:
- Pseudotyping lentiviral vectors with various viral glycoproteins to modulate properties.
- Exploring novel concepts and approaches to overcome gene transfer restrictions.
Main Results:
- Pseudotyping influences vector physicochemical properties, immune interaction, and host range.
- Preliminary in vivo evaluations show promising results for novel lentiviral vector strategies.
Conclusions:
- Lentiviral vectors face limitations in transducing quiescent cells like hematopoietic stem cells.
- Further research and development are crucial for advancing lentiviral vector technology for in vivo gene therapy.