Related Experiment Video
Updated: Aug 26, 2026

Rapid Quantification of Oxidized and Reduced Forms of Glutathione Using Ortho -phthalaldehyde in Cultured Mammalian Cells In Vitro
Published on: June 28, 2024
[The role of glutathione and glutathione-related enzymes in antioxidative process]
1Zakładu Toksykologii, Akademii Medycznej w Białymstoku. toxic@amb.edu.pl
Abstract:
Reactive oxygen species (ROS) are produced within cells during oxidative metabolism. ROS are scavenged by both enzymatic and nonenzymatic antioxidant systems. Reduced glutathione, cysteine-containing tripeptide, and glutathione-related enzymes play a key role in antioxidative processes. Glucose-6-phosphate dehydrogenase (G6PDH), an enzyme of pentose phosphate pathway, is responsible for the regeneration of G6PDH, the main cellular reductant. Recent investigations suggest that G6PDH is essential to control intracellular reductive potential by increasing glutathione intracellular level, which in turn decreases the amount of ROS.
More Related Videos
Related Concept Videos
Phase II Reactions: Glutathione Conjugation and Mercapturic Acid Formation
Several distinctive characteristics distinguish glutathione conjugation from other phase II...
Sulfur Assimilation
Redox Reactions
Redox Reactions
Oxidation of Phenols to Quinones
o-hydroxy phenols are oxidized to o-quinones and p-hydroxy phenols to p-quinones. Such redox reactions involve the transfer of two electrons and two protons. The reversible redox property is crucial in...
Enzymes
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...

