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Updated: Aug 26, 2026

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects
Published on: April 21, 2022
[Effect of selection techniques on chromosome and ultrastructure of human spermatozoa]
Qiaoyu Zhang1, Rong Qin, Qing Chang
1Department of Obstetrics and Gynecology, Southwest Hospital, Third Military University, Chongqing 400038, China.
Objective:
To know the effect of different selection techniques on chromosome abnormality rate and the percentage of spermatozoa with relatively intact head ultrastructure in human sperm.
Methods:
The selection techniques of swim-up, Percoll density gradient centrifugation and doule-flection tube were used to observe sperm chromosome abnormality rate, sex chromosomal ratio and the percentage of spermatozoa with relatively intact head ultrastructure.
Results:
The sperm chromosome abnormality rate, sex chromosomal ratio and the percentage of spermatozoa with relatively intact head ultrastructure remained unchanged after the application of the three selection techniques(P > 0.05).
Conclusion:
The three selection techniques used in this study neither increase the damage to sperm ultrastructure nor exert effects on human chromosome.
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