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Updated: Aug 26, 2026

In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells
Published on: May 16, 2011
Adherence of actinobacillus pleuropneumoniae serotype 1 to swine buccal epithelial cells involves fibronectin
Roberto Hamer-Barrera1, Delfino Godínez, V Idalia Enríquez
1Departamento de Biología Celular, Centro de Investigación y de Estudios Avanzados del IPN, Ap. postal 14-740, México DF 07000, México.
Abstract:
The swine pathogen Actinobacillus pleuropneumoniae serotype 1 was investigated for its ability to adhere to swine, rat, and human buccal epithelial cells (BEC). The highest number of bacteria adhered was to swine BEC. This binding ability was affected by heating, extreme pH, treatment with sodium dodecyl sulfate, ethylenediamine tetra-acetate, or periodate, and proteolysis, suggesting that cell-surface glycoproteins participate in adherence and that adherence is based mostly on ionic interactions. Mannose and swine fibronectin may play a direct role in this interaction. Convalescent-phase serum from naturally infected pigs inhibited the adhesion. There was a correlation between bacterial pathogenicity as well as host specificity and the capacity for adherence to swine BEC. Adhesion to swine BEC provides a convenient method to study in vitro the adherence of A. pleuropneumoniae and other pathogens of the pig respiratory tract.
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