Amygdala inactivation blocks expression of conditioned memory modulation and the promotion of avoidance and freezing

Rats were exposed to shock-paired cues immediately after training on an appetitive preference task. Elevated levels of freezing in and active avoidance of the shock-paired compartment were observed, and memory for the appetitive task was improved when tested 24 hr later. Intra-amygdala muscimol injected before the posttraining exposure eliminated freezing, avoidance, and memory modulation. The blockade of both freezing and active avoidance, which involve competing behavioral tendencies, makes it unlikely that the amygdala itself generates either behavior. The elimination of conditioned memory modulation suggests that conditioned neurohormonal responses were blocked. These conditioned internal responses may comprise the intervening variable of "conditioned fear" and may promote observable behaviors, the form of which is determined by the environment in which they occur.