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Related Experiment Videos

Studies of epidermal lipids using electron microscopy.

D C Swartzendruber1

  • 1Marshall Dermatology Research Laboratories, Department of Dermatology, University of Iowa College of Medicine, Iowa City 52242.

Seminars in Dermatology
|June 1, 1992
PubMed
Summary
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Osmium tetroxide and ruthenium tetroxide are complementary reagents for the preparation of epidermal samples for transmission electron microscopy.

The Journal of investigative dermatology·1995

Ruthenium tetroxide fixation revealed continuous lipid envelopes and lamellae in stratum corneum, advancing understanding of epidermal lipid ultrastructure in mammals.

Area of Science:

  • Dermatology
  • Cell Biology
  • Microscopy

Background:

  • The stratum corneum's lipid structure is crucial for skin barrier function.
  • Previous studies utilized freeze-fracture, but visualizing intercellular lamellae remained challenging.

Purpose of the Study:

  • To visualize intercellular lipid lamellae in stratum corneum using ruthenium tetroxide fixation.
  • To elucidate the ultrastructure of epidermal lipids and their organization.

Main Methods:

  • Ruthenium tetroxide fixation for electron microscopy.
  • Thin sectioning of stratum corneum from three mammalian species, including humans.

Main Results:

  • Demonstrated a continuous lipid envelope surrounding each stratum corneum cell.

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  • Observed lipid lamellae throughout the stratum corneum, appearing as multiple bilayer pairs.
  • Proposed a unique pattern of intervening lipid monolayers between bilayers, potentially aiding stratum corneum cohesion.

    • Conclusions:

    • Ruthenium tetroxide fixation provides detailed visualization of stratum corneum lipid organization.
    • The findings reveal a consistent lamellar structure and lipid envelope in mammalian stratum corneum.
    • Further refinement of the technique is needed to correlate lamellar patterns with permeability changes in disease states.