Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sialyllactose-binding modified DNA aptamer bearing additional functionality by SELEX.

Mohammad Mehedi Masud1, Masayasu Kuwahara, Hiroaki Ozaki

  • 1Department of Applied Chemistry, Gunma University, Kiryu, Gunma 376-8515 Japan.

Bioorganic & Medicinal Chemistry
|February 26, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Conventional Versus Underwater Endoscopic Mucosal Resection for Superficial Non-Ampullary Duodenal Epithelial Tumors ≤ 20 mm: Study Protocol for a Multicenter Randomized Controlled Trial (D-CURE Trial).

Methods and protocols·2026
Same author

Comparison of Automated Point-of-Care Gram Stainer (PoCGS<sup>®</sup>) and Manual Staining.

Diagnostics (Basel, Switzerland)·2025
Same author

Aptamer-based biosensors for wastewater surveillance of influenza virus, SARS-CoV-2, and norovirus: A comprehensive review.

Water research·2025
Same author

ARCaDia: single-round screening of a DNA-type targeted covalent binder possessing a latent warhead.

Chemical communications (Cambridge, England)·2024
Same author

Computer Modelling Study of Volume Kinetics in Intraocular Segments Following Airbag Impact Using Finite Element Analysis.

Clinical ophthalmology (Auckland, N.Z.)·2024
Same author

Long-Term Observation of Prognostic Factors and Clinical Outcome of Vernal Keratoconjunctivitis in Childhood.

Clinical ophthalmology (Auckland, N.Z.)·2024

Researchers developed a novel DNA aptamer that binds to sialyllactose, a molecule crucial for viral infections. This positively-charged aptamer shows potential for diagnostic and therapeutic applications targeting sialyllactose-mediated processes.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • Sialyllactose is a key molecule on cell surfaces involved in viral infections.
  • Developing specific binding agents for sialyllactose is important for diagnostics and therapeutics.

Purpose of the Study:

  • To create a novel, modified DNA aptamer with high binding affinity for sialyllactose.
  • To investigate the structural basis for the aptamer-sialyllactose interaction.

Main Methods:

  • Magnetic-particle-based SELEX (Systematic Evolution of Ligands by Exponential Enrichment) was employed for aptamer selection.
  • Structural analysis of selected aptamers to understand their binding mechanisms.

Main Results:

  • A cationic-charged modified DNA aptamer targeting sialyllactose was successfully developed after 13 SELEX rounds.

Related Experiment Videos

  • The aptamer forms a three-way junction structure, featuring modified thymidines with a positively-charged amino group.
  • The optimized aptamer exhibited a dissociation constant (Kd) of 4.9 µM for sialyllactose.

    • Conclusions:

    • The novel cationic DNA aptamer demonstrates significant binding capability for sialyllactose.
    • The positively-charged modifications and three-way junction structure are crucial for effective sialyllactose binding.
    • This aptamer holds promise for applications in detecting or neutralizing sialyllactose-related biological processes, including viral infections.