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Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations
Published on: March 29, 2017
Simultaneous detection of seven mutations with seven forward primers and one common reverse primer in a single PCR
Qiang Wang1, Qiuyun Liu, Bao-jian Li
1Biotechnology Research Center and Key Laboratory of Gene Engineering of Ministry of Education, School of Life Sciences, Zhongshan University, Canton 510275, PR China.
Journal of Biochemical and Biophysical Methods
|February 26, 2004
Abstract:
A new PCR method is described here to amplify simultaneously several fragments sharing the same template in a single PCR step with a hemi-primer. Using this method, we successfully detected various point mutations in tissue plasminogen activator (tPA) mutants.
