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Size-exclusion chromatography-a review of calibration methodologies
Leopold K Kostanski1, Douglas M Keller, Archie E Hamielec
1Chemical Engineering Department, McMaster University, 1280 Main Street West, Hamilton, Ontario, Canada L8S 4L7. kostans@mcmaster.ca
Journal of Biochemical and Biophysical Methods
|February 26, 2004
Abstract:
Recent developments are reviewed in size exclusion chromatographic calibration methodologies, including direct calibration by using narrow and broad polymer standards and various instrumental methods (nuclear magnetic resonance, mass spectrometry, light scattering) as well as universal calibration with and without viscometry detectors, for simple and complex polymers.